National fruit fly surveillance programme

This annual report summarizes the results of the 2021-2022 National Fruit Fly Surveillance Programme (NFFSP) in New Zealand. The report shows that despite the challenges posed by the Covid-19 pandemic, the programme was successful in meeting its objectives. A total of 139 individual trap runs were used to service the 7878 Lynfield traps in use, with no new traps established but several relocated to improve coverage. From the 2587 trap-run submissions, a total of 8183 vials were submitted, and no exotic fruit flies were detected. Thirteen samples collected in fruit-fly traps were categorized as "specimens of interest," while 9 specimens were submitted by trappers as passive surveillance samples. All lure batches tested during the season met the required standard, and field checks were made to ensure that all lures sent to trappers had been calibrated within the last 12 months. The report concludes that the trapping network was effective in supporting New Zealand's claims of area freedom.

The microbial-killing Ag nanoparticles in food supply chain: How it was applied and what a consumer should know?

Moving to 133 years of the synthesis of citrate-stabilized silver nanoparticles (AgNPs) by M. C. Lea (published in Am. J. Sci, 1889), a myriad of scholarly works and patents were published globally demonstrating the applicability of this microbial-killing nanoparticles in various industries. One of the favorite applications is on the food supply chain whereby AgNPs serve to improve food safety and quality. In this paper, the adaptation of AgNPs in each phase of a typical food supply chain is disclosed, doubts associated with the potential risks brought by this technology, and what a consumer shall be aware of are highlighted.

Comprehensive Overview of Diamide Derivatives Acting as Ryanodine Receptor Activators.

The world's hunger is continuously rising due to conflicts, climate change, pandemics (such as the recent COVID-19), and crop pests and diseases. It is widely accepted that zero hunger is impossible without using agrochemicals to control crop pests and diseases. Diamide insecticides are one of the widely used green insecticides developed in recent years and play important roles in controlling lepidopteran pests. Currently, eight diamine insecticides have been commercialized, which target the insect ryanodine receptors. This review summarizes the development and optimization processes of diamide derivatives acting as ryanodine receptor activators. The review also discusses pest resistance to diamide derivatives and possible solutions to overcome the limitations posed by the resistance. Thus, with reference to structural biology, this study provides an impetus for designing and developing diamide insecticides with improved insecticidal activities.

The 25 countries that could beat malaria by 2025

Speed read WHO draws list of 25 countries targeting malaria elimination by 2025 Eight countries were certified malaria-free last year despite COVID-19 But disruptions to health services during the pandemic could threaten progress The World Health Organization (WHO) says it has identified 25 countries with the potential to stamp out malaria in the next five years, despite the added burden of COVID-19 and antimalarial drug resistance. “No matter what burden of malaria you have in your country, there’s a path that can be walked and the end stage of that path will be eliminating malaria,” Pedro Alonso, director, WHO Global Malaria Programme Last year alone eight countries reported zero indigenous cases of malaria, “a remarkable achievement in view of the ongoing global COVID-19 pandemic,” the report says. About one third of countries around the world reported disruptions in malaria prevention, diagnosis and treatment services during the first quarter of 2021, a survey by the organisation found, while lockdowns and restrictions on movement led to delays in the delivery of insecticide-treated mosquito nets and indoor insecticide spraying campaigns.

Don’t lose focus on diseases of poverty in COVID-19 crisis

Scientists in Africa, Asia and Europe from a range of disciplines have been researching these diseases of poverty as part of Zoonoses and Emerging Livestock Systems (ZELS), a United Kingdom Department for International Development (DFID) and UK Research and Innovation funded programme. Managing zoonoses A finding that treating cattle with insecticides to combat ticks and tsetse flies can contain the risk of sleeping sickness in people offers the possibility of simple and cost-effective disease-management strategies. Discovering that the majority of schistosomiasis transmission and sickness in Senegal and Niger is driven not by a human schistosome species, as had been previously assumed, but through schistosome species from people and their livestock combining to form highly transmissible viable parasitic hybrids, has helped ensure the WHO embraces a One Health approach to eliminating of a disease that infects more than 240 million people globally.

Scientists eye gene drive technology to combat malaria

Speed read Innovation must be ramped up to tackle malaria, scientists urge Gene drive technology could reduce mosquito populations Pace of malaria innovation has slowed, say experts Scientists are hoping that adoption of gene drive technology could reduce mosquito populations as they call for new innovations in the fight against malaria, a fatal disease widespread in Sub-Saharan Africa. The World Health Organization (WHO) says the Africa region accounted for around 94 per cent of all global malaria cases and deaths in 2019. Fredros Okumu, entomologist and director of science, Ifakara Health Institute, Tanzania “It is a cost-effective way to cut down malaria vectors, and is simple to implement because the mosquitoes themselves do the work,” said Birungi during a Roll Back Malaria Partnership virtual boot camp on malaria control innovation on 15 November.

In Silico Study and Excito-Repellent Activity of Vitex negundo L. Essential Oil against Anopheles gambiae

(1) Background: Essential oil from Vitex negundo is known to have repellent and insecticidal properties toward the Anopheles gambiae and this is linked to its monoterpene and sesquiterpene content. In this work, an effort is made to delineate the constitution of V. negundo essential oil (VNEO) and their interaction with odorant-binding proteins (OBPs) of A. gambiae and hence access its repellent efficiency as cost-effective and safer malaria vector control alternatives. (2) Methods: Anopheles species authentication was performed by genomic DNA analysis and was subjected to behavioral analysis. GC-MS profiling was used to identify individual components of VNEO. Anopheles OBPs were obtained from the RCSB protein data bank and used for docking studies. Determination of ligand efficiency metrics and QSAR studies were performed using Hyper Chem Professional 8.0.3, and molecular dynamics simulations were performed using the Desmond module. (3) Results: GC-MS analysis of VNEO showed 28 compounds (monoterpenes, 80.16%;sesquiterpenes, 7.63%;and unknown constituents, 10.88%). The ligand efficiency metrics of all four ligands against the OBP 7 were within acceptable ranges. β-selinene (−12.2 kcal/mol), β-caryophellene (−9.5 kcal/mol), sulcatone (−10.9 kcal/mol), and α-ylangene (−9.3 kcal/mol) showed the strongest binding affinities for the target proteins. The most stable hydrophobic interactions were observed between β-selinene (Phe111 and Phe120), Sulcatone (Phe54 and Phe120), and α-ylangene (Phe111), while only sulcatone (Tyr49) presented H-bond interactions in the simulated environment. (4) Conclusions: Sulcatone and β-caryophyllene presented the best log p values, 6.45 and 5.20, respectively. These lead phytocompounds can be used in their purest as repellent supplement or as a natural anti-mosquito agent in product formulations.

The COVID-19 Pandemic: An Opportunity to Assess the Global Health Status

According to WHO: "As long as one child is infected all countries are at risk to get 200,000 new cases per year" [6, 7]. * TB claims 1.5 million lives each year. Three million cases were missed by the detection systems and the funding to combat the disease was back to the 2016 level that year [8-10]. * The number of cholera cases remains high and many are not reported. [...]it can be re-introduced into many countries like it was in Haiti in the 2010 [11-13]. * Due to the sylvatic cycle in Africa yellow fever cannot be realistically eradicated [14-17]. * Because of the cattle and wild animal reservoirs of Trypanosoma rhodesiense in East Africa, sleeping sickness cannot be practically eradicated. * In 2007, WHO announced a renewed strategy to eliminate Chagas' disease by 2010. The main problems linked to the elimination of malaria have not been tackled like: deforestation, agricultural expansion, infrastructure development, the biological differences in Anopheles species adapted to different landscapes, human and mosquito migrations, travelers, climate change [24-26]. * The discovery of a dog-fish cycle in Chad renders a lasting elimination of dracunculiasis improbable [27-28]. Most worrisome are the trends for the diseases mentioned above and there is no sign of imminent or short term eradication. [...]lack of capacity is the main obstacle to adequate healthcare in developing countries [30]. * Quantitatively, data are dismal [31]. * Quality wise, the gap is huge and increasing [32]. * Unfortunately, the evolution in various places is not toward improvement particularly in Sub-Saharan Africa. * Questions have not been raised on the unreliable origin and misutilization of resources.

New insights on malaria

Malaria is long known as a deadly vector borne infection, caused by five parasite species of the coccidian genus Plasmodia that are present in as many as 85 countries. Despite significant progresses have been achieved to control the infection by early diagnosis and artemisinin combination treatment, insecticide-treated nets and indoor residual spraying, malaria still represents a major public health issue in many endemic low-income countries. New diagnostic tools of higher sensitivity and specificity are now available for use in endemic countries to better guide diagnosis and treatment. In particular, highly sensitive rapid antigenic tests are now available and the loop-mediated isothermal amplification is a very promising and highly sensitive diagnostic tool. After 2015, decreasing morbidity and mortality trends have been stagnating because of limited funding, emergence of parasite and vector resistance to drugs and insecticides respectively and, recently, by the disrupting effect of COVID-19 pandemic. The incomplete knowledge of the complex immunity of malaria infection has slowed the development of an effective vaccine. However, in 2021, the RTS-S vaccine, however of suboptimal protective efficacy, has been made available for routine use in children above 5 months of age. Population movements has increased the chance of observing imported malaria in non-endemic areas, where malaria competent vectors may still exist.

When Scent Becomes a Weapon—Plant Essential Oils as Potent Bioinsecticides

Crop protection still mostly relies on synthetic pesticides for crop pest control. However, the rationale for their continued use is shaded by the revealed adverse effects, such as relatively long environmental persistence that leads to water and soil contamination and retention of residues in food that brings high risks to human and animal health. As part of integrated pest management, biopesticides may provide crop protection, being eco-friendly and safe for humans and non-target organisms. Essential oils, complex mixtures of low-molecular-weight, highly volatile compounds, have been highlighted as major candidates for plant-derived bioinsecticides that are up to the sustainable biological standard. In this review, we screened the insecticidal activity of essential oils or their purified compounds, with focus given to their modes of action, along with the analyzed advantages and problems associated with their wider usage as plant-derived insecticides in agriculture.

Status of Pesticide Usage on Golf Courses in Korea and Optimal Pesticide Usage Plan

Risks to human health and the environment owing to pesticide usage have arisen interest, increasing the demand for reducing pesticide consumption used on golf courses. However, standard guidelines or manuals for reducing pesticide usage on golf courses in Korea are lacking. Herein, the trends of pesticides on golf courses were investigated, and the optimal pesticide usage plan was proposed for continuous pesticide reduction. In 2019, there were 539 golf courses in Korea. With the increasing number of golf courses in 2010–2019, pesticide usage increased continuously. Fungicides accounted for more than half the pesticides used, followed by insecticides and herbicides. Except for golf courses that do not employ chemical pesticides, pesticide usage per unit area varied in the range of 0.02–65.81 kg ha−1 (average of 6.97 kg ha−1). In the US, best management practices and integrated pest management (IPM) have been stipulated and are operational in each state for pesticide management in golf courses, recognizing chemical pesticide usage to be the last approach for pest control and turf health maintenance. Considering that Korea globally ranks 10th in the number of golf facilities and courses, the establishment of IPM practices suitable for domestic conditions is essential.

UHPLC-OrbiTrap MS Characterization of Phenolic Profiles in French Marigold Extracts and Analysis of Their Antifeedant Activity against Colorado Potato Beetle.

French marigold is an aromatic plant rich in polyphenolic secondary metabolites, which pesticidal potential was examined in this study. Ultra-high-performance liquid chromatography (UHPLC) connected with OrbiTrap mass spectrometer (MS) identified 113 phenolics and revealed the most detailed phytochemistry of French marigold published so far. Depending on plant material (flowers or leaves) and solvents used for extraction (water, methanol, dichloromethane), the phenolic composition varied. Methanol extract of flowers, with 89 identified phenolics and high antioxidant activity statistically comparable with positive control Trolox, was chosen for testing of antifeedant potential against the 3rd and 4th instars of Colorado potato beetle (CPB). A significant reduction in final body mass of 4th larval stage fed with potato leaves coated with methanol extract of flowers in the concentration of 10 mg/mL was observed (157.67 mg vs. 182.26 mg of controls fed with non-treated leaves). This caused delayed molting since treated larvae reached the maximal mass a day after controls and this delay persisted during the entire larval development. Continuous feeding caused a 25% decline in digestive proteolytic activity of the 4th instar in comparison to controls. The results suggest that French marigold methanol extract of flowers could be proposed as a promising antifeedant for CPB management, with an impact on the reduction in the environmental footprint associated with synthetic pesticide application.

Prospects of the Application of Garlic Extracts and Selenium and Silicon Compounds for Plant Protection against Herbivorous Pests: A Review

Protection of plants against herbivorous pests is an important aspect that guarantees agricultural efficiency, i.e., food provision to populations. Environmental, water and foodstuff pollution by toxic pesticides, along with climate changes, highlight the necessity to achieve intensive development of ecologically safe methods of herbivory control. This review discusses modern methods of plant protection against insect pests: the biofortification of plants with selenium, treatment of plants with bulk and nano-silicon, and utilization of garlic extracts. The peculiarities of such methods of defense are described in relation to growth stimulation as well as increasing the yield and nutritional value of products. Direct defense methods, i.e., mechanical, hormonal, through secondary metabolites and/or mineral element accumulation, and indirect defense via predator attraction are discussed. Examples of herbivorous pest control during plant growth and grain/seed storage are emphasized. A comparison of sodium selenate, silicon containing fertilizer (Siliplant) and garlic extract efficiency is analyzed on Raphanus sativus var. lobo infested with the cruciferous gall midge Contarinia nasturtii, indicating the quick annihilation of pests as a result of the foliar application of garlic extract or silicon-containing fertilizer, Siliplant.

World malaria report 2021

The World Health Organization (WHO) World Malaria Report 2021 < https://www.who.int/publications/i/item/9789240040496 > estimates that there were 241 million malaria cases, including 627,000 deaths, worldwide in 2020, which represents around 14 million more cases, and 69,000 more deaths, than 2019. Approximately two-thirds of these additional deaths were linked to disruptions in the provision of malaria prevention, diagnosis and treatment during the COVID-19 pandemic. Sub-Saharan Africa continues to carry the heaviest malaria burden, accounting for about 95% of all cases and 96% of all deaths in 2020, with around 80% of deaths in the region among children under five years old. Since 2015, the baseline date for the WHO's global malaria strategy, registered increases in malaria deaths were reported in 24 countries. In the 11 countries that carry the highest burden of malaria worldwide, cases increased from 150 million in 2015 to 163 million cases in 2020, and malaria deaths increased from 390,000 to 444,600 over that same period. As in previous years, the report includes an up-to-date assessment on the burden of malaria at global, regional, and country levels, and tracks investment in malaria programmes and research, as well as detailing progress across the four intervention areas of prevention, diagnosis, treatment and surveillance. There are also dedicated chapters on malaria elimination and key threats, such as insecticide and drug resistance.

Animal toxins - Nature's evolutionary-refined toolkit for basic research and drug discovery.

Venomous animals have evolved toxins that interfere with specific components of their victim's core physiological systems, thereby causing biological dysfunction that aids in prey capture, defense against predators, or other roles such as intraspecific competition. Many animal lineages evolved venom systems independently, highlighting the success of this strategy. Over the course of evolution, toxins with exceptional specificity and high potency for their intended molecular targets have prevailed, making venoms an invaluable and almost inexhaustible source of bioactive molecules, some of which have found use as pharmacological tools, human therapeutics, and bioinsecticides. Current biomedically-focused research on venoms is directed towards their use in delineating the physiological role of toxin molecular targets such as ion channels and receptors, studying or treating human diseases, targeting vectors of human diseases, and treating microbial and parasitic infections. We provide examples of each of these areas of venom research, highlighting the potential that venom molecules hold for basic research and drug development.